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Real-Time Virology Assays

바이오테크랩 xCELLigence Cell Impedance

Measure Viral Cytopathic Effects (CPE) in Real Time Real-Time Virology Assays

Developing novel methods to screen antivirals is important for creating broad-spectrum antiviral drugs and vaccines. The accurate measurement of viral activity plays an essential role for viral vaccine development and infectious disease studies. When infected with a virus, host cells often display changes that are collectively referred to as a cytopathic effect (CPE). These effects include cell shrinkage or enlargement, deterioration/lysis, cell fusion, and the formation of inclusion bodies. Not all viruses cause a CPE in their host cell, but if they do, it can be a useful tool for various research applications from virus titer determination to the detection and quantification of neutralizing antibodies.

Explore Agilent xCELLigence Real-Time Cell Analysis technologies to learn about a viral cytopathic effect (CPE) assay that provides:

  • Rapid and highly sensitive kinetic data for the entire virus life cycle

  • A simple and high-throughput workflow, without the use of agar or labels

  • Reduced workload and manual handling of infectious samples through automation

  • More reproducible data by eliminating the subjectivity of visual interpretation

Fully Investigate Antiviral Drug Effects for the Entire Viral Life Cycle

Obtain highly sensitive and quantitative measurements of viral cytopathic effects, inside your incubator, with xCELLigence RTCA eSight. eSight provides multiplexed live cell imaging and impedance readouts that provide deeper insights into antiviral drug mechanism of action and capture cellular details spanning activation of host cell antiviral pathways, apoptosis, and barrier function disruption. Ensure crucial data and time points are never missed as you fully investigate drug impact over the entirety of the viral life cycle.

Multiplex Impedance-Based Data with Live Cell Imaging for Multiple Perspectives

바이오테크랩 xCELLigence Cell Impedance

While the standard xCELLigence RTCA instruments (SP, MP, HT models) provide comprehensive, non-invasive and label-free analysis in 96- or 384-well formats, the xCELLigence RTCA eSight adds live cell imaging for increased confidence in your cell analysis and conclusions. In one instrument, capture five different perspectives (impedance, brightfield, red, green, and blue fluorescence imaging) on the exact same population of cells to reveal greater insight into viral cytopathic effects.

Screen and Characterize Virus-Neutralizing Antibodies

바이오테크랩 xCELLigence Cell Impedance

An antibody’s ability to bind a purified antigen does not always correlate with its ability to “neutralize” the virus and block infection. In this example, xCELLigence RTCA impedance technology shows how a soluble CAR is unable to neutralize a virus when using 40k cells/well and the high virus concentration of MOI = 222. In contrast, when using 40k cells/well with the low virus concentration of MOI = 0.4, the soluble CAR completely blocks the ability of adenovirus-GFP to kill the host cells.

Real-Time Virology Assays


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